VanRx_Mike said:COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…
Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight. This confirms identity — not just purity.
Why this matters: HPLC can show you a "pure" compound, but if it's pure of the WRONG compound, purity is meaningless. Mass spec at m/z 4113.58 Da confirms it's actually semaglutide. My Janoshik report includes both HPLC and mass spec.
If your testing provider only offers HPLC, consider upgrading to include mass spec for at least your first batch from a new vendor.
ben_calgary said:One addition: an unstated method makes a report close to uninterpretable.
Genuinely useful, thank you. I had the facts and not the framework.
VanRx_Mike said:COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…
Vendor quality matters when discussing certificates of analysis. I've tested my last 3 batches through Janoshik and purity has ranged from 99.0% to 99.0%. Consistency is key.
For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.
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Shop Reference StandardsSkepticalSean said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 16.8 minutes (consistent with semaglutide reference standard)
Peak purity: 98.5%
Related substances: 0.7% (single impurity at 10.2 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.5 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
Moderator note: the sourcing question belongs in the vendor section and has been split out. Report rather than reply if it drifts again.