Dr.LipidDallas said:They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
SkepticalSean said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
I tested my certificates of analysis batch through Janoshik — 99.2% purity. That's within acceptable range for compounded peptides. Turnaround was 14 business days.
The test costs ~$134 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.
Dr.BariatricHTX said:I am more sceptical of certificates as a category than this thread is.
COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:
- No HPLC data: If there's no chromatography graph, it's not a real COA
- Exact 100% purity: Suspicious — real testing always shows slight variation
- No batch number: Can't trace back to specific production
- No endotoxin testing: For injectables, this is essential
- Template looks like it was made in Word: Professional labs have standardized formats
I have seen fake COAs that are literally photoshopped. Always verify independently if possible.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsA narrower follow-up, since the general answer is now clear:
Why two laboratories report different purity for the same batch, and which number to believe?
Closing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.