From the other side of the consultation, briefly.
Third-party testing services for certificates of analysis besides Janoshik:
- Janoshik: Gold standard for peptide community, ~$142, 10-14 day turnaround
- Valitech: US-based, ISO 17025 accredited, ~$212
- Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
- ChemTox: Basic identity and purity testing, ~$112
Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.
LabKate said:Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight.
That reframing is the part I needed. Sending this to two other people who asked me the same thing last week.
Adding the clinical framing, because it changes how the question reads.
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:
- Purity %: Should be >97%. Below 95% is concerning.
- Retention time: Should match reference standard for the peptide
- Related substances: Degradation products should be <2% total
- Single impurity: No individual impurity should exceed 1%
My last batch: 99.6% purity, 0.2% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsDr.Martinez said:HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…
Same experience, arrived at from the opposite direction.