newstart_MO said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Third-party testing services for certificates of analysis besides Janoshik:
- Janoshik: Gold standard for peptide community, ~$136, 10-14 day turnaround
- Valitech: US-based, ISO 17025 accredited, ~$246
- Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
- ChemTox: Basic identity and purity testing, ~$116
Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.
bbq_ray_KC said:Agreed, and mass spec answers a question purity does not: identity.
Bookmarking. The distinction being drawn above is the one nobody else makes.
newstart_MO said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 56 submitted tests:
- 85% tested at ≥98% purity
- 10% tested at 95-98% purity
- 5% tested at <95% purity (concerning)
The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsDr.GastroMayo said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight. This confirms identity — not just purity.
Why this matters: HPLC can show you a "pure" compound, but if it's pure of the WRONG compound, purity is meaningless. Mass spec at m/z 4113.58 Da confirms it's actually semaglutide. My Janoshik report includes both HPLC and mass spec.
If your testing provider only offers HPLC, consider upgrading to include mass spec for at least your first batch from a new vendor.
Moderator note: two posts asking for a source have been merged into one. Please search the thread before asking again. Report rather than reply if it drifts again.