Dr.GastroMayo said:They are not contradictory, they measure different things, and this is the single most common misreading of a certificate.
I am more sceptical of certificates as a category than this thread is. The document tells you about a sample somebody chose to send, at a moment in time, and nothing about the vial in your hand. Independent purchase-and-test is a different quality of evidence and everything else is inference.
anna.melb_AU said:Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.
I tested my certificates of analysis batch through Janoshik — 99.2% purity. That's within acceptable range for compounded peptides. Turnaround was 14 business days.
The test costs ~$140 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.
Dr.CardioMD said:I am more sceptical of certificates as a category than this thread is.
Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 52 submitted tests:
- 85% tested at ≥98% purity
- 10% tested at 95-98% purity
- 5% tested at <95% purity (concerning)
The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference StandardsFollowing on from Dr.MetabolicMD — and this may be the naive question:
Why two laboratories report different purity for the same batch, and which number to believe?
Reporting back.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.