julia.endo said:Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$127, 10-14 day turnaround…
Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 62 submitted tests:
- 82% tested at ≥98% purity
- 12% tested at 95-98% purity
- 6% tested at <95% purity (concerning)
The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.
JennaRN said:Agreed, and mass spec answers a question purity does not: identity.
Thank you for spelling out the reasoning rather than just the conclusion. I will report back once I have actually tried it.
julia.endo said:Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$127, 10-14 day turnaround…
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 15.8 minutes (consistent with semaglutide reference standard)
Peak purity: 98.1%
Related substances: 0.4% (single impurity at 9.6 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.6 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
Sigma-Aldrich — Research-Grade Standards
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Shop Reference StandardsDr.CardioMD said:Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.
Day 1: 99.2% purity
Day 14: 98.3% purity
Day 28: 97.0% purity
Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.